The AOCS Lipid Library

SILVER ION CHROMATOGRAPHY OF LIPIDS

Silver ion chromatography has been one of the more important techniques in the armoury of lipid analysts for nearly 50 years. In these pages, we will review the technique from both theoretical and practical standpoints.

Review of Silver Ion Chromatography

Boryana Nikolova-Damyanova

An important review was published by the Oily Press in 1992, and as it is now out of print they have kindly given us permission to reproduce it on this website -

Nikolova-Damyanova, B. Silver ion chromatography and lipids. In: Advances in Lipid Methodology - One. pp. 181-237 (Ed. W.W. Christie, Oily Press, Ayr) (1992).

• Introduction, and Principles of Silver Ion Complexation with Double Bonds
• Silver Ion Thin-Layer Chromatography
• Silver Ion High-Performance Liquid Chromatography
• Low-Pressure Silver Ion Column Chromatography, and Combined Chromatographic Techniques
• References

Aspects of Silver Ion Chromatography

Theoretical Considerations

• An introduction to silver ion chromatography (by B. Nikolova-Damyanova & W.W. Christie)
• Mechanism of retention of unsaturated lipids (by B. Nikolova-Damyanova)
• Retention order of fatty acids and triacylglycerols (by B. Nikolova-Damyanova)
• Multiple interactions in silver ion chromatography of lipids (by B. Nikolova-Damyanova) - - April 3rd, 2012

Practical Procedures

• How to make a column for silver ion HPLC (by W.W. Christie)
• Solid-phase extraction chromatography in the silver ion mode (by W.W. Christie)

Further original articles will be added here by Bill Christie and Boryana Nikolova-Damyanova as time and other professional commitments permit. A list of references to the use of HPLC in the silver ion mode for analysis of lipids is available here...

The most important recent review on the topic is that published by the Oily Press - Nikolova-Damyanova, B. Lipid analysis by silver ion chromatography. In: Advances in Lipid Methodology - Five. pp. 43-123 (Ed. R.O. Adlof, Oily Press, Bridgwater) (2003).

	Institute of Organic Chemistry, Centre of Phytochemistry, Sofia 1113, Bulgaria and James Hutton Institute (and Mylnefield Lipid Analysis), Invergowrie, Dundee (DD2 5DA), Scotland.
Updated: April 3rd, 2012	Credits/disclaimer		© AOCS