Lipid Compositions of Animal Tissues


1.  Introduction

Comprehensive discussion of the enormous literature on compositions of animal, plant and microbial tissues would be a daunting task, and it is only possible here to summarize briefly some of the more significant features of lipid composition, with some highly selective (and simplified) examples of relevant analyses. Further data are available for specific lipids in most of the webpages in this part of the website.

One problem in comparing data from different sources is the method of presentation, which is often dependent on the nature of the analytical methods used. For example, it is easier technically to analyse the phospholipids and glycolipids following isolation as distinct groups, separately from the simple lipids. Often there is no attempt subsequently to integrate the data. To add to the confusion, results of analyses of simple lipids are often reported in terms of weight percent of each lipid class, since the data are acquired in this form, while the results for phospholipids are most frequently recorded as molar percent, especially when phosphorus analysis is used as the means of quantification. Many of the data listed below are from publications that may appear old but are nonetheless reliable. The results from modern lipidomics analyses are often difficult to summarize in a simple tabular form.


2.  Lipid Class Compositions

In animal tissues, the structural lipid components, such as the phospholipids, tend to be rather constant in composition under the normal physiological conditions so meaningful comparisons between different organs can often be made. On the other hand, the proportions of the simple lipids, especially the triacylglycerols, can vary greatly according to the dietary or physiological state of the animal, and this information is not always recorded in papers. Where possible, data are presented for the liver, because it contains all of the important lipid classes, and because of the central importance of this organ in lipid metabolism. Marine lipids are discussed only briefly here; Ackman has edited a two-volume work on the subject [1].

As examples, some data on the lipid composition of selected tissues from the rat (an important animal for biological experimentation) are shown in Table 1. Those lipids listed are by far the most abundant in these tissues, and they are those most often seen in other organs, although other lipids can assume importance in some circumstances. Lipids present at relatively low concentrations only, such as most of the lysophospholipids, ceramides, sphingosine, sphingosine-1-phosphate were not determined in these analyses, but they are of course of great metabolic importance. Nor were the diacyl, alkylacyl and alkenylacyl forms of the phospholipids distinguished.

Table 1. The composition of the lipid classes (weight % of the total) in rat heart, liver, erythrocytes and plasmaa.
Lipid Class Tissue
heart liver erythrocytes plasma
  cholesterol esters trace 2 - 16
  triacylglycerols 4 7 - 49
  cholesterol 4 5 30 6
  diacylglycerols 1 - trace trace
  free fatty acids - trace - 2
  cardiolipinb 12 5 - -
  phosphatidylethanolamine 33 20 21 -
  phosphatidylinositol 4 4 3 -
  phosphatidylserine - - 3 -
  phosphatidylcholine 39 55 32 24
  sphingomyelin 2 2 8 2
  lysophosphatidylcholine - - 1 1
a Christie, W.W., J. Lipid Res., 26, 507-512 (1985).
b also contains cerebrosides and phosphatidylglycerol

Cholesterol esters, triacylglycerols and free (unesterified) cholesterol tend to be the most abundant simple lipids; when substantial amounts of diacylglycerols and free fatty acids, especially the latter, are encountered in a sample, it is usually indicative of artefactual hydrolysis during storage or extraction of the tissues. The choline-containing constituents, i.e. phosphatidylcholine and sphingomyelin, but predominantly the former, tend to be the most abundant phospholipids (50 to 60% of the total), followed by phosphatidylethanolamine, and then by phosphatidylinositol and phosphatidylserine. The results quoted for rat liver lipids are therefore typical of this and many other organs. Cardiolipin is a major component of mitochondrial lipids in particular and so is found in appreciable amounts in heart muscle; phosphatidylglycerol only appears to assume importance in lung surfactant. Again, when appreciable amounts of phosphatidic acid or lysophospholipids are encountered in samples, artefactual hydrolysis may have occurred during processing or analysis.

Scottish thistleIn erythrocytes, all the lipids appear to be constituents of the membranes, and the results of the analysis listed here indicate that only cholesterol and the phospholipids are present. These data would be typical of many other species, and any departure from this general pattern is symptomatic of an underlying metabolic difference; for example in ruminant erythrocytes, there is virtually no phosphatidylcholine, which is replaced entirely by sphingomyelin. The fatty acid components of erythrocytes are often considered a better marker of long-term nutritional status than are the plasma lipids.

The compositions of the plasma lipids are of particular importance as they supply fatty acids to all tissues, and are assumed to be in some form of compositional equilibrium with them. Of course, biopsy samples of plasma are easy to obtain. Those lipids reported as plasma lipid constituents here are typical; phosphatidylcholine is present as a higher proportion of the total phospholipids than in any other tissue, while cholesterol esters tend to more abundant than in most organs (other than in steroidogenic tissues such as the adrenals). The proportion of triacylglycerols in the plasma in this instance was higher than normal, because an old obese rat was used for the analysis. Lipids are transported in plasma in the form of complexes with proteins, i.e. lipoproteins, which render them compatible with their aqueous environment. It should be recognized that an analysis of the total lipids in plasma can give only part of the picture, and it may be necessary to determine the compositions of each of the lipoprotein fractions before definitive metabolic conclusions can be drawn.

Brain phospholipids are distinctive in that they tend to contain a higher proportion of phosphatidylserine (~10%) than other tissues, and much of the phosphatidylethanolamine especially is in plasmalogen form.

All membranes (indeed each side of the bilayer) in a tissue can have distinctive compositions that are in some way related to their function. The results of some analyses of the phospholipids of the membranes of rat liver are recorded in Table 2. Naturally, the same phospholipids are present as is described above for the intact organ, but the relative proportions vary markedly. For example, cardiolipin and sphingomyelin are much more abundant in mitochondria and plasma membrane, respectively, than in any of the other membranes.

Table 2. The phospholipid composition of whole tissue and membrane preparations from rat liver (mol % lipid phosphorus).
Lipid class Membrane
Whole tissue Nuclei Mitochondria Microsomes Plasma membrane
   [a] [b] [c] [c] [c]
  cardiolipin 5 - 15 2 -
  phosphatidylethanolamine 25 26 34 22 20
  phosphatidylinositol 7 4 7 8 7
  phosphatidylserine 3 6 1 4 4
  phosphatidylcholine 51 57 41 59 43
  sphingomyelin 4 6 2 4 23
  lysophosphatidylcholine 1 - 1 2 2
References: a, Wuthier, R.E. J. Lipid Res., 7, 544-550 (1966). b, Gurr, M.I. et al. Biochim. Biophys. Acta, 106, 357-370 (1965). c, Colbeau, A. et al. Biochim. Biophys. Acta, 249, 462-492 (1971).

The data listed in Table 2 are incomplete, however, as cholesterol is an important constituent of membranes, modifying their fluidity, and the proportion relative to the phospholipids should be determined. In plasma membrane preparations from rat liver, the molar ratio of cholesterol to phospholipids is 0.76, while that in microsomes and mitochondria is 0.1 [2]. In addition, only a part of each phospholipid is the diacyl form and in most tissues, the phosphatidylethanolamine especially tends to contain an appreciable proportion of plasmalogens. On the other hand, the plasmalogen form of phosphatidylcholine appears only to occur in high proportions in heart muscle and reproductive tissues. With a few significant exceptions, the lipid compositions of equivalent membranes from different tissues and even from different species are in general rather similar, probably because these membranes have common functional requirements (though the fatty acid components may differ).


3.  Fatty Acid Compositions of Glycerolipids

The fatty acid composition of each lipid in a tissue is frequently distinctive and can vary markedly between species. It is obviously greatly, but not entirely, dependent on the nature of the diet of the animal concerned (although in many analytical studies, such details are not given). Kuksis [3] has reviewed the fatty acid compositions of animal glycerolipids. Data for the fatty acid compositions of the depot fats, which are highly responsive to dietary influence, in several animal species are listed in Table 3.

Table 3. The fatty acid compositions (mol % of the total) of the adipose tissue lipids of various animal species.
 Fatty acid Species
Rat [a] Pig [b] Sheep [c] Horse [a] Herring [d] Seal [d]
  16:0 23 29 21 26 21 10
  16:1 5 3 2 8 11 16
  18:0 6 18 35 5 - -
  18:1 35 41 31 31 23 26
  18:2 19 8 2 9 1 2
  18:3 2 - - 18
  20:1 10 14
  20:5 9 7
  22:1 10 7
  22:6 5 8

References: a, Brockerhoff, H. et al. Biochim. Biophys. Acta, 116, 67-72 (1966). b, Christie, W.W. and Moore, J.H. Biochim. Biophys. Acta, 210, 46-56 (1970). c, Christie, W.W. and Moore, J.H. J. Sci. Food Agric., 22, 120-124 (1971). d, Brockerhoff, H. and Hoyle, R.J. Arch. Biochem. Biophys., 102, 452-455 (1963).

In all, there are relatively high contents of 16:0 and 18:1 fatty acids. Species differences are most apparent in the relative concentrations of the saturated and polyunsaturated components. In the rat, pig and horse, for example, there are appreciable amounts of linoleic acid; this is a minor component in the sheep, because it is subjected to microbial biohydrogenation in the rumen, and in the herring and seal, because it is present at low levels only in their food chain. Although the sheep and horse may consume a very similar diet, there are high levels of linolenic acid, derived from the herbage, in the lipids of the horse and not in those of the sheep, again because of biohydrogenation in the rumen of the latter. Fish, such as the herring, tend to contain relatively high amounts of C20 and C22 fatty acids, derived from the microflora and microfauna, which they consume. In turn, the composition of the depot fat of the seal reflects its diet of fish.

Each lipid class in a tissue has a distinctive fatty acid composition also, probably related to its function but in a way that is still only partly understood. Some data for the main glycerolipids and the cholesterol esters of rat liver are listed in Table 4.

Table 4. The fatty acid compositions (mol % of the total) of the main glycerolipids and the cholesterol esters of rat liver.
Fatty acid Lipid class
TG [a] PC [a] PE [a] PI [b] PS [c] Cardx [d] CE [e]
  16:0 27 14 18 7 4 7 24
  16:1 4 1 8 3
  18:0 7 34 37 40 47 4 20
  18:1 27 10 8 3 3 20 15
  18:2 12 12 5 3 2 59 16
  20:4(n-6) 1 20 23 40 25 2 14
  22:6(n-3) - 4 7 2 16 - -
Abbreviations: TG, triacylglycerols; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; Card, cardiolipin; CE, cholesterol esters.
        x of the mitochondrial fraction
References: a, Wood, R. and Harlow, R.D. Arch. Biochem. Biophys., 131, 495-501 (1969). b, Holub, B.J. and Kuksis, A. J. Lipid Res., 12, 699-705 (1971). c, Holub, B.J. and Kuksis, A. Adv. Lipid Res., 16, 1-125 (1978). d, Colbeau, A. et al. Biochim. Biophys. Acta, 249, 462-492 (1971). e, Connellan, J.M. and Masters, C.J. Biochem. J., 94, 81-84 (1965).

In essence, only C16 and C18 fatty acids are found in significant amounts in the triacylglycerols, although most of the glycerophospholipids contain substantial proportions of the longer-chain polyunsaturated components. For example, the phosphatidylcholine contains 50% of saturated fatty acids, while arachidonic acid constitutes 20% of the total. The phosphatidylethanolamine has a similar proportion of saturated fatty acids, but somewhat less linoleic acid and correspondingly more of the C20 and C22 polyunsaturated fatty acids. Characteristically, high proportions of stearic and arachidonic acids are present in the phosphatidylinositol, while the composition of the phosphatidylserine is similar except that the 22:6(n-3) fatty acid substitutes for part of the arachidonic acid. The cardiolipin differs markedly from all the other glycerophospholipids in that the single fatty acid, linoleic acid, comprises nearly 60% of the total. The composition of the cholesterol esters tends to resemble that of the phosphatidylcholine, but there is somewhat less stearic and more palmitic acid in the former. Similar general compositional trends are seen, although the absolute values may differ, in comparing the same lipids in other tissues and, with dietary influences superimposed, in comparing the corresponding lipids of other species. On the other hand, brain phospholipids are again distinctive in that they contain much higher proportions of docosahexaenoic acid, especially in the phosphatidylethanolamine.


4.  Fatty Acid Distributions within Glycerolipid Molecules

In addition to each lipid class in a tissue having a distinctive fatty acid composition, each position of the glycerol moiety tends to have a unique fatty acid composition that is determined during the biosynthesis of a lipid mainly by the specificities of various acyltransferases. Again, the positional distributions of fatty acids in particular lipid classes can vary markedly between tissues and species and can have some metabolic importance. Data for triacylglycerols [4] and for glycerophospholipids [5] have been reviewed. Some results for the principal glycerolipids of rat liver, taken from a single systematic analytical study [6], are listed in Table 5.

Table 5. Positional distributions of fatty acids in each position (sn-1, 2 or 3) of the principal glycerolipids of rat liver (results are expressed as mol % of the total in each position) [6].
Fatty acid Lipid class and position
  triacyl-sn-glycerol phosphatidyl-
choline
phosphatidyl-
ethanolamine
sn-1 sn-2 sn-3 sn-1 sn-2 sn-1 sn-2
  16:0 57 13 12 23 6 25 11
  16:1 4 3 4 1 1 trace trace
  18:0 9 4 8 65 4 65 8
  18:1 22 58 61 7 13 8 8
  18:2 4 19 11 1 23 - 10
  20:4(n-6) - 1 1 trace 39 - 46
  22:6(n 3) - 7 - 13

In the triacylglycerols, the highest proportion of the 16:0 is in position sn-1, but the other saturated fatty acid, 18:0, is distributed between positions sn-1 and sn-3 mainly. The 16:1 is found in equal abundance in all three positions, but the C18 unsaturated fatty acids are distributed mainly between positions sn-2 and sn-3. In the phosphatidylcholine, much of the saturated fatty acids are in position sn-1, although a significant relative proportion of the 16:0 is also found in position sn-2. Some of the 18:1 is in position sn-1, but virtually all of the remaining unsaturated fatty acids are in position sn-2. Similar relative distributions are seen in the phosphatidylethanolamine, except that a slightly greater proportion of the saturated fatty acids are present in position sn-2. 1,2-Diacyl-sn-glycerols are a common intermediate in the biosynthesis of each of these lipid classes, but there is little correspondence in the compositions of this part of the molecules, when the phospholipids are compared with the triacylglycerols.

In addition, analyses of the molecular species distributions in the three classes of lipids indicated that there was little in common between the structures of the triacylglycerols and those of the phospholipids [6]. This suggests that there is great selectivity for particular molecular species of the intermediate diacylglycerols, utilized for the synthesis of each lipid class, or that some hydrolysis and re-acylation of lipids occurs to give the final compositions (the "Lands' cycle").

Scottish thistleMuch of the published work on the structures of animal triacylglycerols has been concerned with those of adipose tissue, which tends to contain most of the body stores of fat in the form of this single lipid class. The results quoted here for rat liver triacylglycerols are not dissimilar to those obtained for adipose tissue for many animal species. In most instances, position sn-1 contains appreciable amounts of the saturated fatty acids, position sn-2 contains mainly unsaturated and any shorter-chain fatty acids, and position sn-3 consists predominantly of unsaturated and longer-chain fatty acids. The principal exception to this type of distribution was thought to be the triacylglycerols of the pig and related species, in which position sn-2 is occupied largely by palmitic acid (70% or more), but increasing numbers of tissues containing triacylglycerols with a structure of this kind are being revealed as research progresses. For example, milk fats from all the higher mammals studied have more than half of their total content of palmitic acid in position sn-2, and the same is true of the triacylglycerols of lymph, plasma and adrenals in ruminant animals. Another interesting feature of milk fats, especially those of ruminants, is that all the short-chain fatty acids (4:0 and 6:0 specifically) are located exclusively in position sn-3. A number of excellent analytical studies of the molecular species distributions in animal triacylglycerols have been published, but as the results are not easily summarized, readers are referred to a specialist review for detailed information [4].

The positional distributions of fatty acids in each of the two most abundant phospholipids of rat liver, as listed in Table 5, are typical for these lipids in many other tissues and species. In some tissues, the proportions of saturated fatty acids in position sn-2 can be somewhat higher, but polyunsaturated fatty acids never appear to occur in appreciable amounts in position sn-1. Perhaps the best-known example of a glycerophospholipid containing a high proportion of saturated fatty acids is the phosphatidylcholine of lung surfactant, of which up to 60% can be the dipalmitoyl form. The distribution of fatty acids in phosphatidylinositol follows the common pattern, and a high proportion consists of the 1-stearoyl-2-arachidonoyl molecular species.


5.  Sphingolipid Compositions

The sphingolipid components of many tissues and species have been analysed, but those of bovine kidney have been studied in detail with respect both to the relative amounts in various regions of the organ and to the nature of the lipid and non-lipid portions of the molecules [7,8]. A few of the results obtained are listed in Table 6 for illustrative purposes.

Table 6. The sphingolipid composition of different bovine kidney regions (results expressed as mg lipid per gram dry tissue) [7].
  Lipid class Cortex Medulla Whole tissue
  ceramides 1.0 0.7 -
  glucosylceramides 0.6 0.6 0.8
  galactosylceramides 0.2 1.3 0.4
  di- and triglycosyl-
    ceramides
0.3 0.7 0.4
  sulfatides 0.1 0.9 -
  sphingomyelins 17.8 9.8 14.4

Sphingomyelin is by far the most abundant sphingolipid, but significant amounts of ceramides and mono-, di- and triglycosylceramides, and sulfatides are present also. The relative proportions of each vary appreciably at different sites in the kidney, presumably because of the differing functions of the membranes in each region.

The fatty acid composition of the sphingomyelin fraction of rat liver is listed in Table 7 [9]. In comparison to the glycerophospholipids, a very different range of fatty acids is seen. For example, virtually no long-chain polyunsaturated fatty acids are found in sphingomyelin (and other sphingolipids, although they are sometimes incorrectly identified as constituents), but C20 to C24 saturated and monoenoic fatty acids, with odd or even chain-lengths, are present in addition to the normal C16 and C18 components. A similar range of fatty acid constituents is seen in most other sphingolipids, and the bimodal chain-length distribution often causes individual sphingolipids separated by chromatographic means to appear as two adjacent bands. In addition, sphingoglycolipids frequently contain a similar range of fatty acids to that listed, but with a hydroxyl substituent in position 2.

Table 7. The fatty acid composition (weight % of the total) in the sphingomyelin of rat liver, and the long-chain base composition (weight % of the total) of ceramide from human liver.
Rat liver sphingomyelin [9] Human liver ceramide [7]
fatty acid % long-chain base          %          
16:0 22 d16:0 trace
18:0 10 d17:0 trace
18:1 4 d16:1 5
18:2 2 d18:0 2
20:0 2 d17:1 3
22:0 14 d18:1(i) a 2
23:0 9 d18:1 77
24:0 24 d18:2 8
24:1 13 d19:1(ai) 3
a abbreviations: i, iso-methyl branch; ai, anteiso-methyl branch

The long-chain base composition of ceramide, which is probably similar to that of sphingomyelin, from human liver is also listed in Table 7. Sphingosine accounts for 77% of the total, but a number of other bases are present in small amounts. Relatively simple base compositions of this kind are found in the other sphingolipids of tissues from most simple-stomached animals. In ruminants, on the other hand, the long-chain base compositions can be much more complex, and may include trihydroxy bases.


6.  Lipidomic Analyses

As cautioned earlier, it is not easy to present the results of modern lipidomics analyses in a concise tabular fashion. However, it would be remiss not to point readers to a paper from the LipidMaps consortium, which I suspect is destined to become a classic, i.e. an analysis of the lipids of macrophages [10]. It shows what can be achieved when a large dedicated team tackles a problem with modern mass spectrometric methodology. Information on the techniques of lipidomics is available in our webpages here..


References

  1. Ackman, R.G. (Editor) Marine Biogenic Lipids, Fats and Oils (two volumes) (CRC Press, Boca Raton, FL) (1990).
  2. Colbeau, A., Nachbaur, J. and Vignais, P.M. Enzymic characterization and lipid composition of rat liver subcellular membranes. Biochim. Biophys. Acta, 249, 462-492 (1971).
  3. Kuksis, A. Fatty acid composition of glycerolipids of animal tissues. In: Handbook of Lipid Research Vol. 1. Fatty Acids and Glycerides, pp. 381-442 (ed. A. Kuksis, Plenum Press, New York) (1978).
  4. Christie, W.W. The positional distribution of fatty acids in triglycerides. In: Analysis of Oils and Fats, pp. 313-339 (ed. R.J. Hamilton and J.B. Rossell, Elsevier Applied Science Publishers, London) (1986).
  5. Holub, B.J. and Kuksis, A. Metabolism of molecular species of diacylglycerophospholipids. Adv. Lipid Res., 16, 1-125 (1978).
  6. Wood, R. and Harlow, R.D. Structural studies of neutral glycerides and phosphoglycerides of rat liver. Arch. Biochem. Biophys., 131, 495-501 (1969).
  7. Karlsson, A.A. Analysis of intact polar lipids by high-pressure liquid chromatography-mass spectrometry/tandem mass spectrometry with use of thermospray or atmospheric pressure ionization. In: Lipid Analysis in Oils and Fats, pp. 290-316 (edited by R.J. Hamilton, Blackie, London) (1998).
  8. Karlsson, A.A., Arnoldsson, K.C., Westerdahl, G. and Odham, G. Common molecular species of glucosyl ceramides, lactosyl ceramides and sphingomyelins in bovine milk determined by high-performance liquid chromatography-mass spectrometry. Milchwissenschaft, 52, 554-559 (1997).
  9. Fex, G. Metabolism of phosphatidyl choline, phosphatidyl ethanolamine and sphingomyelin in regenerating rat liver. Biochim. Biophys. Acta, 231, 161-169 (1971).
  10. Dennis, E.A. and 27 others. A mouse macrophage lipidome. J. Biol. Chem., 285, 39976-39985 (2010) (DOI: 10.1074/jbc.M110.182915).

Acknowledgement: This document is based on part of Chapter 1 of the Third edition of Lipid Analysis by the author and published by P.J. Barnes & Associates (The Oily Press Ltd). It was omitted from the Fourth (and latest) edition of the book to save space.


W.W. Christie

James Hutton Institute (and Mylnefield Lipid Analysis), Invergowrie, Dundee (DD2 5DA), Scotland.

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