GAS CHROMATOGRAPHY AND LIPIDS

The following web pages in HTML format are the text of the book "Gas Chromatography and Lipids" by William W. Christie and published in 1989 by the Oily Press Ltd. The book is now out of print, and is republished here in an html version by kind permission of P.J. Barnes & Associates (The Oily Press), who retain the copyright.

You can obtain a PDF file of the book as a free download from the Oily Press Ltd.

Part 1. An Introduction to Lipids and Gas Chromatography

	Chapter 1.	Introduction and Summary
	Chapter 2.	Fatty acids and Lipids: Structures, Extraction and Fractionation into Classes
		Sections A to D. Lipid structures Sections E and F. Extraction and fractionation into lipid classes
	Chapter 3.	Gas chromatography: Theoretical Aspects and Instrumentation

Part 2. The Analysis of Fatty Acids

	Chapter 4.	The Preparation of Derivatives of Fatty Acids
	Chapter 5.	Gas Chromatographic Analysis of Fatty Acid Derivatives
		Sections A to D. Instrumentation and identification via retention data Sections E to G. Applications to natural samples and quantification
	Chapter 6.	Isolation of Fatty Acids and Identification by Spectroscopic and Chemical Degradative Techniques
		Sections A and B. Isolation of fatty acids Sections C and D. Identification by spectroscopy and chemical degradation
	Chapter 7.	Gas Chromatography-Mass Spectrometry and Fatty Acids

Part 3. The Analysis of Lipids other than Fatty Acids

	Chapter 8.	Gas Chromatographic Analysis of Molecular Species of Lipids
		Sections A and B. Introduction and high-temperature gas chromatography of triacylglycerols Sections C to G. GC of complex glycerolipids and other simple lipids Sections H and I. GC of sphingolipids and of total lipid extracts
	Chapter 9.	Alternative or Complementary Methods for the Analysis of Molecular Species of Lipids
	Chapter 10.	Some Miscellaneous Separations of Lipids by Gas Chromatography

		References

Please note! Many of the procedures used in the analysis of lipids require the use of solvents and other hazardous materials. We cannot accept any responsibility for accidents that may occur while using methods described here.

In preparing this book for web publication, I have been pleasantly surprised by how much is still relevant today. On the other hand, most of the text referring to packed columns is now redundant, and many of the commercial products, such as particular GC or HPLC phases, are no longer manufactured although equivalents may be available. HPLC, especially linked to mass spectrometry, has made enormous strides in recent years. My latest book (co-authored with Xianlin Han) "Lipid Analysis - 4th Edition" (2010), also published by the Oily Press, is right up-to-date as well as being much more comprehensive in terms of applications to lipids in general.

William W. Christie, James Hutton Institute (and Mylnefield Lipid Analysis), Invergowrie, Dundee (DD2 5DA), Scotland.
Author	Updated: July 12^th, 2011	Credits/disclaimer	AOCS

The AOCS Lipid Library

GAS CHROMATOGRAPHY AND LIPIDS