Literature Survey

The Evaporative Light-Scattering Detector in Lipid Analysis

Because of its universality and the ease with which it handles complex solvent gradients, the evaporative light-scattering detector has greatly aided many aspects of lipid analysis. For convenience, references to the newer charged-aerosol detector are also listed here. I can recommend two review articles cited below  (Christie (1992) and Moreau & Christie (1999)). The first of these is now available online here.

  • Christie,W.W. Detectors for high-performance liquid chromatography of lipids with special reference to evaporative light-scattering detection. In: 'Advances in Lipid Methodology - One' (edited by W.W. Christie, Oily Press, Ayr), pp. 239-271 (1992).
  • Moreau, R.A. and Christie, W.W. The impact of evaporative light-scattering detectors on lipid research. INFORM, 10, 471-478 (1999).

The following references were collected as part of our regular literature reviews for our own research purposes. We cannot claim to have covered the subject exhaustively, but we have done our best. References are listed alphabetically by the first author of a paper, but not necessarily chronologically by that author. This list will be updated from time to time, please check at the bottom of the page for when this was last done.

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  • Abidi, S.L., Mounts,T.L. and Finn,T. A preferred solvent system for high-performance liquid-chromatographic analysis of soybean phospholipids with evaporative light-scattering detection. J. Am. Oil Chem. Soc., 73, 535-536 (1996).
  • Abidi, S.L., Mounts,T.L. and Rennick,K.A. Separations of major soybean phospholipids on beta-cyclodextrin-bonded silica. J. Liqu. Chromatogr., 17, 3705-3725 (1994).
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  • Alvarez, J.G., Slomovic,B. and Ludmir,J. Analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by high-performance liquid chromatography. J. Chromatogr. B, 690, 338-342 (1997).
  • Andersson, M.B.O. and Blomberg,L.G. A miniaturized evaporative light scattering detector for application with packed microcolumn high-performance liquid chromatography. J. Microcolumn Sep., 10, 249-254 (1998).
  • Anklam, E., Lipp,M. and Wagner,B. HPLC with light-scattering detector and chemometric data evaluation for the analysis of cocoa butter and vegetable fats. Fett-Lipid, 98, 55-59 (1996).
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  • Arborati, M., Benchorba,D., Lesieur,I., Bizot-Espiard,J.G., Guardiola-Lemaitre,B., Chapman,M.J. and Ninio,E. Oxidative degradation of cholesteryl esters in low-density lipoproteins: Analysis by liquid chromatography-light scattering and protection by a new synthetic antioxidant, S20478. Fund. Clin. Pathol., 11, 68-77 (1997).
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  • Blaas, N. and Humpf, H.U. Structural profiling and quantitation of glycosyl inositol phosphoceramides in plants with Fourier transform mass spectrometry. J. Agric. Food Chem., 61, 4257-4269 (2013).
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  • Brouwers,J.F.H.M., Vernooij,E.A.A.M., Tielens,A.G.M. and van Golde,L.M.G. Rapid separation and identification of phosphatidylethanolamine molecular species. J. Lipid Res., 40, 164-169 (1999).
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  • Byrdwell, W.C. ’Dilute-and-shoot’ triple parallel mass spectrometry method for analysis of vitamin D and triacylglycerols in dietary supplements. Anal. Bioanal. Chem., 401, 3317-3334 (2011)
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  • Caboni, M.F., Iafelice,G., Pelillo,M. and Marconi,E. Analysis of fatty acid steryl esters in tetraplold and hexaploid wheats: Identification and comparison between chromatographic methods. J. Agric. Food Chem., 53, 7465-7472 (2005).
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  • Caboni, M.F., Menotta,S. and Lercker,G. High-performance liquid chromatography separation acid light-scattering detection of phospholipids from cooked beef. J. Chromatogr. A, 683, 59-65 (1994).
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  • Cascone, A., Eerola,S., Ritieni,A. and Rizzo,A. Development of analytical procedures to study changes in the composition of meat phospholipids caused by induced oxidation. J. Chromatogr. A, 1120, 211-220 (2006).
  • Chamorro, L., Garcia-Cano, A., Busto, R., Martinez-Gonzalez, J., Albillos, A., Lasuncion, M.A. and Pastor, O. Quantitative profile of lipid classes in blood by normal phase chromatography with evaporative light scattering detector: Application in the detection of lipid class abnormalities in liver cirrhosis. Clin. Chim. Acta, 421, 132-139 (2013).
  • Charlesworth, J.M. Evaporative analyser as a mass detector for HPLC. Anal. Chem., 50, 1414-1420 (1978).
  • Christie, W.W. Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection. J. Lipid Res., 26, 507-512 (1985).
  • Christie, W.W. Chromatographic analysis of phospholipids. Z. Lebensm. Unters. Forsch., 181, 171-182 (1985).
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  • Christie, W.W. Separation of molecular species of triacylglycerols by HPLC with a silver ion column. J. Chromatogr. A, 454, 273-284 (1988).
  • Christie, W.W. Some recent advances in the chromatographic analysis of lipids. Analusis, 26, M34-M40 (1998).
  • Christie, W.W. Recent developments in high-performance liquid and gas chromatography of lipids. Rev. Franc. Corps Gras, 38, 155-160 (1991).
  • Christie, W.W. Detectors for high-performance liquid chromatography of lipids with special reference to evaporative light-scattering detection. In: 'Advances in Lipid Methodology - One' (edited by W.W. Christie, Oily Press, Ayr), pp. 239-271 (1992).
  • Christie, W.W. and Breckenridge,G.H.M. Separation of cis and trans isomers of unsaturated fatty acids by HPLC in the silver ion mode. J. Chromatogr. A, 469, 261-269 (1989).
  • Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition), 446 pages (Oily Press, Bridgwater, U.K. and Woodhead Publishing Ltd, Cambridge, U.K.) (2010) - Woodhead Publishing Ltd.
  • Christie, W.W., Brechany,E.Y. and Shukla,V.K.S. Analysis of seed oils containing cyclopentenyl fatty acids by combined chromatographic procedures. Lipids, 24, 116-120 (1989).
  • Christie, W.W., Brechany,E.Y. and Stefanov,K. Silver ion high-performance liquid chromatography and gas chromatography-mass spectrometry in the analysis of complex fatty acid mixtures: application to marine invertebrates. Chem. Phys. Lipids, 46, 127-136 (1988).
  • Christie, W.W., Noble,R.C. and Davies,G. Phospholipids in milk and dairy products. J. Soc. Dairy Technol., 40, 10-12 (1987).
  • Christie, W.W., Gill,S., Nordback,J., Itabashi,Y., Sanda,S. and Slabas,A.R. New procedures for rapid screening of leaf lipid components from Arabidopsis. Phytochem. Anal., 9, 53-57 (1998).
  • Christie, W.W. and Morrison,W.R. Separation of complex lipids of cereals by HPLC with mass detection. J. Chromatogr. A, 436, 510-513 (1988).
  • Christie, W.W. and Stefanov,K. Separation of picolinyl ester derivatives of fatty acids by high-performance liquid chromatography for identification by mass spectrometry. J. Chromatogr. A, 392, 259-265 (1987).
  • Christie, W.W. and Urwin,R.A. Separation of lipid classes from plant tissues by HPLC on chemically bonded stationary phases. J. High Resolut. Chromatogr., 18, 97-100 (1995).
  • Cocks, S. and Smith,R.M. Analysis for fatty acid methyl esters by using supercritical fluid chromatography with mass evaporative light scattering detection. Anal. Proc. (London), 28, 11-12 (1991).
  • Conforti, F.D., Harris,C.H. and Rinehart,J.T. HPLC analysis of wheat-flour lipids using an evaporative light-scattering detector. J. Chromatogr. A, 645, 83-88 (1993).
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Updated: February 5th, 2014