Gas Chromatography and Lipids

The following web pages in HTML format are the text of the book Gas Chromatography and Lipids by William W. Christie and published in 1989 by the Oily Press Ltd. The book is now out of print, and is republished here in an html version by kind permission of P.J. Barnes & Associates (The Oily Press), who retain the copyright.

Part 1.  An Introduction to Lipids and Gas Chromatography
  Chapter 1. Introduction and Summary
  Chapter 2. Fatty acids and Lipids: Structures, Extraction and Fractionation into Classes
    Sections A to D.  Lipid structures
Sections E and F.  Extraction and fractionation into lipid classes
  Chapter 3. Gas chromatography: Theoretical Aspects and Instrumentation 
 
Part 2.  The Analysis of Fatty Acids
  Chapter 4. The Preparation of Derivatives of Fatty Acids
  Chapter 5. Gas Chromatographic Analysis of Fatty Acid Derivatives
    Sections A to D.  Instrumentation and identification via retention data
Sections E to G.  Applications to natural samples and quantification
  Chapter 6. Isolation of Fatty Acids and Identification by Spectroscopic and Chemical Degradative Techniques
    Sections A and B.  Isolation of fatty acids
Sections C and D.  Identification by spectroscopy and chemical degradation
  Chapter 7. Gas Chromatography-Mass Spectrometry and Fatty Acids
 
Part 3.  The Analysis of Lipids Other Than Fatty Acids
  Chapter 8.  Gas Chromatographic Analysis of Molecular Species of Lipids
    Sections A and B.  Introduction and high-temperature gas chromatography of triacylglycerols
Sections C to G.  GC of complex glycerolipids and other simple lipids
Sections H and I.  GC of sphingolipids and of total lipid extracts
  Chapter 9. Alternative or Complementary Methods for the Analysis of Molecular Species of Lipids
  Chapter 10.  Some Miscellaneous Separations of Lipids by Gas Chromatography
 
    References

 

Please note! Many of the procedures used in the analysis of lipids require the use of solvents and other hazardous materials. We cannot accept any responsibility for accidents that may occur while using methods described here.

In preparing this book for web publication, I have been pleasantly surprised by how much is still relevant today. On the other hand, most of the text referring to packed columns is now redundant, and many of the commercial products, such as particular GC or HPLC phases, are no longer manufactured although equivalents may be available. HPLC, especially linked to mass spectrometry, has made enormous strides in recent years. My latest book (co-authored with Xianlin Han) "Lipid Analysis - 4th Edition" (2010), also published by the Oily Press, is right up-to-date as well as being much more comprehensive in terms of applications to lipids in general.

Updated August 27, 2012